Journal of Ethno-Pharmaceutical Products

Investigating the possibility of squalene production by some medicinal plants using thin-layer chromatography (TLC)

Document Type : Research Paper

Authors

Mahmood Maleki 1
Omolbanin Nezhad Dehbakri 2
Davoud Darvishi Zeidabadi 3
Shahryar Shakeri 4

1 Dept. of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

2 MSc student of agricultural biotechnology, Graduate University of Advanced Technology, Kerman, Iran

3 Forests and Rangelands Research Department, Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.

4 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

https://doi.org/10.48306/epp.2023.2002740.1056
Abstract
Medicinal plants contain active ingredients in one or some of their organs. Squalene is one of the active ingredients that prevent heart attacks and cardiovascular diseases and protect the body from some cancers. The aim of the present study was to investigate the presence of squalene in a number of medicinal plants. In this experiment, the plant oils were extracted and measured using Bligh & Dyer with minor changes. TLC (thin layer chromatography) was used to identify squalene. Comparison of TLC of standard squalene with TLC of the investigated medicinal plant samples showed that Caryophillium aromaticus, Descurainia sophia, Portulaca Oleracea, Papaver somniferum and Nigella Sativa contained squalene. Although the percentage of Papaver somniferum and Nigella Sativa seed oil was higher than other medicinal plants, the squalane spot of clove plant had a higher intensity of color and this indicates a higher concentration of squalene in this plant.

Keywords

Medicinal plant
Oil extraction
Thin Layer Chromatography

Subjects

Journal of Ethno-Pharmaceutical Products
Volume 3, Issue 1
September 2023
Pages 24-30

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