Document Type : Research Paper
Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman-Iran
Background: Trachyspermum ammi and Foeiniculum vulgare (Apiaceae) have been widely employed in traditional medicine to treat many diseases. Methods: The cytotoxic activity of methanolic extracts and essential oils of T.ammi and F.vulgare seeds on gastric cancer cell line (AGS) and Human Skeletal Muscle cell line (HSkMC) were assessed by MTT method. In vitro toxicity was also evaluated on Artemia salina. The antibacterial activity was measured by the Microtiter broth dilution method. Result: The proliferation of cancer cells was inhibited by methanolic extracts and essential oils. The results showed a greater degree of cytotoxicity on AGS at the dose of 400μg/mL of methanolic extracts and essential oils of T.ammi and F.vulgare with IC50 values lower than 50 µg/mL at 48 to 72h. so, they can be considered appropriate for further purification and are agree with the US National Cancer Institute. The methanolic extracts and essential oils exhibited cytotoxicity activity against brine shrimp larvae (LC50: 1066.4 and 137.5 µg/mL for T.ammi) and (LC50: 1267.5 and 235.7 µg/mL for F.vulgare). The antimicrobial activity of methanolic extracts and essential oils showed maximum inhibitory activity against S.aureus with MICs (0.35 and 0.08 fold) and (0.45 and 0.1 fold) for T.ammi and F.vulgare, respectively. Conclusion: Therefore, our results showed that the methanolic extracts and essential oils of T.ammi and F.vulgare have antiproliferation and antibacterial properties and could be used as adjuvant therapy against common gastric and pathogenic bacteria.
- Ashraf M. Salt tolerance of cotton, some new advances. Crit Rev Plant Sci. 2002; 2(1): 1-30.
- Zarshenas MM, Moein M, Samani SM, Petramfar P. An overwiew on Ajwain (Trachyspermum ammi) pharmacological effects; modern and traditional. J Nat Rem. 2014; 14(1), 98-105.
- Omer EA, Said-Al Ahl HAH, El Gendy AG. Yield and essential oil of Ajwain (Trachyspermum ammi) plant cultivated in saline soil of north Sinai in Egypt. J Essent Oil-Bear Plants. 2014;17: 469-477.
- Moein MR, Zomorodian K, Pakshir K, Yavari F, Motamedi M, Zarshenas MM. Trachyspermum ammi (L.) sprague: chemical composition of essential oil and antimicrobial activities of respective fractions. J Evid Based complementary Altern Med. 2015;20(1):50-56.
- Singh V, Ali M, Saxena R, Shekhar C, Anand D. Volatile constituents and antimicrobial and antifungal activities of immature green seeds of Trachyspermum ammi Linn. J Essent Oil-Bear Plants. 2008; 11:120-123.
- Hussein G, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K. Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus (HCV) protease. Phytother Res. 2000; 14(7):510-516.
- Singh K, Singh DK. Effect of different combinations of MGK-264 or piperonyl butoxide with plant derived molluscicides on snail reproduction. Arch Environ Contam Toxicol. 2000; 38;182-190.
- Javed I, Iqbal Z, Rahman ZU, Khan FH, Muhammad F, Aslam B, Ali L. Comparative antihyperlipidaemic efficacy of Trachyspermum ammi extracts in Albino rabbits. Pak Vet J 2006; 26(1): 23-29.
- Lateef M, Iqbal Z, Akhtar MS, Jabbar A, Khan MN, Gilani AH. Preliminary screening of Trachyspermum ammi (L.) seed for anthelminthic activity in sheep. Trop Anim Health Prod. 2006;.38(6): 491-96.
- Park IK, Kim J, Lee SG, Shin SC. Nematicidal activity of plant essential oils and components from Ajowan (Trachyspermum ammi), Allspice (Pimenta dioica) and Litsea (Litsea cubeba) Essential Oils Against Pine Wood Nematode (Bursaphelenchus xylophilus). J Nematol. 2007; 39(3): 275-279.
- Mathew N, Misra-Bhattacharya S, Perumal V, Muthuswamy K. Antifilarial lead molecules isolated from Trachyspermum ammi. Molecules. 2008; 13(9): 2156-68.
- Chaubey MK. Fumigant toxicity of essential oils from some common spices against pulse beetle, Callosobruchus chinensis (Coleoptera: Bruchidae). J Oleo Sci. 2008; 57: 171-179.
- Kaur GJ, Arora DS. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Altern Med 2009; 9(1): 30.
- Pandey SK, Upadhyay S, Tripathi AK. Insecticidal and repellent activities of thymol from the essential oil of Trachyspermum ammi (Linn) Sprague seeds against Anopheles stephensi. Parasitol Res. 2009; 105(2): 507-512.
- Chatterjee S, Goswami N, Kothari N. Evaluation of antioxidant activity of essential oil from Ajwain (Trachyspermum ammi) seeds. Int J Green Pharm. 2013;7: 140–144.
- Gandomi H, Abbaszadeh S, JebelliJavan A, Sharifzadeh A. Chemical Constituents, Antimicrobial and Antioxidative Effects of T rachyspermum ammi Essential Oil. J Food Process Preserv. 2014;38(4):1690-5.
- Ebeed NM, Abdou HS, Booles HF, Salah SH, Ahmed ES, Fahmy Kh. Antimutagenic and chemoprevention potentialities of sweet fennel (Foeniculum vulgare Mill.) hot water crude extract. J Am Sci. 2010; 6(9): 831-42
- Parejo I, Jauregui O, Sanchez-Rabaneda F, Viladomat F, Bastida J, Codina C. Separation and characterization of phenolic compounds in fennel (Foeniculum vulgare) using liquid chromatography-negative electrospray ionization tandem mass spectrometry. J Agric Food Chem. 2004; 52(12): 3679–3687.
- Ozbek H, Ugras S, Dulger H. Hepatoprotective effect of Foeniculum vulgare essential oil. Fitoter. 2003; 74(3): 317-319.
- Kaileh M, Berghe WV, Boone E, Essawi T, Haegeman G. Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J Ethnopharmacol. 2007; 113(3):510-516.
- Modaress NV, Asadipour M. Comparison of the effectiveness of fennel and mefenamic acid on pain intensity in dysmenorrhoea. East Mediterr Health J. 2006; 12(3-4): 423-427.
- Badgujar SB, Patel VV, Bandivdekar AH. Foeniculum vulgare Mill: A Review of Its Botany, Phytochemistry, Pharmacology, Contemporary Application, and Toxicology. Biomed Res Int. 2014; 2014:32.
- Choi EM, Hwang JK. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia. 2004; 75(6): 557-565.
- Joshi H, Parle M. Cholinergic basis of memory/strengthening effects of Foeniculum vulgare Linn. J Med Food. 2006; 9(3):413-17.
- Tognolini M, Ballabeni V, Bertoni S, Bruni R, Impicciatore M, Barocelli E. Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis. Pharmacol Res. 2007; 56(3): 254-260.
- Barros L, Heleno SA, Carvalho AM, Ferreira IC. Systematic evaluation of the antioxidant potential of different parts of Foeniculum vulgare Mill. from Portugal. Food Chem. Toxicol. 2009; 47(10): 2458-2464.
- Nickavar B, Abolhasani FA. Screening of antioxidant properties of seven Umbelliferae fruits from Iran. Pak J Pharm Sci. 2009; 22(1): 30-35.
- Mohamad RH, El-Bastawesy AM, Abdel-Monem MG, Noor AM, Al-Mehdar HAR, Sharawy SM, El-Merzabani MM. Antioxidant and anticarcinogenic effects of methanolic extract and volatile oil of fennel seeds (Foeniculum vulgare). J Medicinal Food. 2011; 14(9):986-01.
- Rahamouz-Haghighi S, Bagheri K, Danafar H, Sharafi A. Anti-proliferative properties, biocompatibility, and chemical composition of different extracts of Plantago major medicinal plant. Iran Biomed J. 2021;25(2):106.
- Rahamouz-Haghighi S, Bagheri K, Sharafi A, Danafar H. Establishment and elicitation of transgenic root culture of Plantago lanceolata and evaluation of its anti-bacterial and cytotoxicity activity. Prep Biochem Biotechnol. 2021;51(3):207-224.
- Rajkumar V, Gunjan G, Ashok Kumar R, Lazar M. Evaluation of cytotoxic potential of Acorus calamus rhizome. Ethnobotanical Leaflets. 2009; 13:832-839.
- Singh B, Kale RK. Chemomodulatory effect of Trachyspermum ammi on murine skin and forestomach papillomagenesis. Nutr Cancer. 2010; 62(1): 74-84.
- Ramya N, Priyadharshini XX, Prakash R, Dhivya R. Anti-cancer activity of Trachyspermum ammi against MCF7 cell lines mediates by p53 and Bcl-2 mRNA levels. J Phytopharmacol. 2017; 6:78-83.
- Abdel-Hameed ES, Bazaid SA, Al Zahrani O, El-Halmouch Y, El-Sayed MM, El-Wakil E. Chemical composition of volatile components, antimicrobial and anticancer activity of n-hexane extract and essential oil from Trachyspermum ammi L. Seeds Orient J Chem. 2014;30(4):1653-1662.
- Khorsandi K, Kianmehr Z, Ghelichkhani E. Combination effect of red light irradiation and Traychspermum ammi essential oil on colorectal cancer cells (SW480). Lasers Med Sci. 2021; 30:1-10.
- Vitali LA, Beghelli D, Nya PC, Bistoni O, Cappellacci L, Damiano S, Lupidi G, Maggi F, Orsomando G, Papa F, Petrelli D. Diverse biological effects of the essential oil from Iranian Trachyspermum ammi. Arabian J Chem. 2016;9(6):775-86.
- Zidorn C, Jöhrer K, Ganzera M, Schubert B, Sigmund EM, Mader J, Greil R, Ellmerer EP, Stuppner H. Polyacetylenes from the Apiaceae vegetables carrot, celery, fennel, parsley, and parsnip and their cytotoxic activities. J Aagric Food Chem. 2005; 53(7):2518-23.
- Birdane FM, Cemek M, Birdane YO, Gulcin I, Buyukokuroglu E. Beneficial effects of vulgare ethanol-induced acute gastric mucosal injury in rat. World J Gastroenterol. 2007; 13(4): 607–661.
- Pradhan M, Sribhuwaneswari S, Karthikeyan D, Minz S, Sure Pi, Chandu A.N, Mishra U, Kamalakannan K, Saravanankumar A, Sivakumar T. In-vitro cytoprotection activity of Foeniculum vulgare and Helicteres isora in cultured human blood lymphocytes and antitumour activity against B16F10 melanoma cell line. Res J Pharm Technol. 2008; 1(4): 450–2.
- Rahamooz-Haghighi S, Asadi MH, Akrami H, Baghizadeh A. Anti-carcinogenic and anti-angiogenic properties of the extracts of Acorus calamus on gastric cancer cells. Avicenna J Phytomedicine. 2017;7(2):145.
- Rahamooz-Haghighi S, Asadi MH. Anti-proliferative effect of the extracts and essential oil of Pimpinella anisum on gastric cancer cells. J HerbMed Pharmacol; 2016;5(4).
- Ruebhart DR, Wickramasinghe W, Cock IE. Protective efficacy of the antioxidants vitamin E and Trolox against Microcystis aeruginosa and microcystin-LR in Artemia franciscana nauplii. J Toxicol Environ Health Part A. 2009;72(24):1567–75.
- Ahmad I, Mehmood J, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol. 1998; 62(2):183-193.
- Patel JD, Patel DK, Shrivastava A, Kumar V. Screening of plant extracts used in traditional antidiarrhoeal medicines against pathogenic Escherichia coli. Sci World J. 2008; 6(6): 63-67.
- Kaur GJ, Arora DS. In vitro antibacterial activity of three plants belonging to the family Umbelliferae. Int J Antimicrob Agents. 2008; 31(4):393-395.
- Mahady GB, Pendland SL, Stoia A, Hamill FA, Fabricant D, Dietz BM, Chadwick LR. In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastro- intestinal disorders. Phytother. Res. 2005; 19(11): 988-991.
- Cwikla C, Schmidt K, Matthias A, Bone KM, Lehmann R, Tiralongo E. Investigations into the antibacterial activities of phytothera-peutics against Helicobacter pylori and Campylobacter jejuni. Phytother Res. 2009; 24(5): 649-656