Journal of Ethno-Pharmaceutical Products

Antiproliferative and antibacterial properties of methanolic extract and essential oil of Trachyspermum ammi and Foeniculum vulgare seeds on gastric cancer, Artemia salina larvae and pathogenic bacteria.

Document Type : Research Paper

Authors

Samaneh Rahamouz-Haghighi 1
malek hossein asadi 2
amin baghizadeh 3

1 Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

3 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman-Iran

Abstract
Background: Trachyspermum ammi and Foeiniculum vulgare (Apiaceae) have been widely employed in traditional medicine to treat many diseases. Methods: The cytotoxic activity of methanolic extracts and essential oils of T.ammi and F.vulgare seeds on gastric cancer cell line (AGS) and Human Skeletal Muscle cell line (HSkMC) were assessed by MTT method. In vitro toxicity was also evaluated on Artemia salina. The antibacterial activity was measured by the Microtiter broth dilution method. Result: The proliferation of cancer cells was inhibited by methanolic extracts and essential oils. The results showed a greater degree of cytotoxicity on AGS at the dose of 400μg/mL of methanolic extracts and essential oils of T.ammi and F.vulgare with IC50 values lower than 50 µg/mL at 48 to 72h. so, they can be considered appropriate for further purification and are agree with the US National Cancer Institute. The methanolic extracts and essential oils exhibited cytotoxicity activity against brine shrimp larvae (LC50: 1066.4 and 137.5 µg/mL for T.ammi) and (LC50: 1267.5 and 235.7 µg/mL for F.vulgare). The antimicrobial activity of methanolic extracts and essential oils showed maximum inhibitory activity against S.aureus with MICs (0.35 and 0.08 fold) and (0.45 and 0.1 fold) for T.ammi and F.vulgare, respectively. Conclusion: Therefore, our results showed that the methanolic extracts and essential oils of T.ammi and F.vulgare have antiproliferation and antibacterial properties and could be used as adjuvant therapy against common gastric and pathogenic bacteria.

Keywords

Ajwain
brine shrimp
Fennel
MTT assay
MIC value

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Journal of Ethno-Pharmaceutical Products
Volume 2, Issue 2
September 2021
Pages 42-50

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