Journal of Ethno-Pharmaceutical Products

Study the effect of salt stress on antioxidant compounds of artichoke under invitro conditions

Document Type : Research Paper

Authors

Masome Rahimbareshi 1
Azim Ghasemnezhad 1
Mehdi Alizadeh 2
Ahad Yamochi 2

1 Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract
Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ‎ingredient is concerned by pharmaceutical industry. Abiotic stresses including salinity, ‎changes the metabolic pathways of cultured cells and leads to the production of secondary ‎metabolites. This study was aimed to determine the effect of salt stress on antioxidant ‎compounds of callus focusing on the phenol, flavonoid, and chlorogenic acid and caffeic acid ‎production under in vitro conditions. The present study was carried out as two separate ‎experiments as direct culture (cultured explant) on MS solid medium with different salinity ‎concentrations (0, 50, 150, 300, 600, 1200 µM NaCl) and indirect culture (callus culture) in ‎different salin concentrations of 0, 50, 150, 300, 600, 1200, 2000, 4000, 6000 and 75000 µM ‎NaCl. Four weeks after culture the mentioned compounds were measured via distinctive ‎methods. Results showed that, versus to indirect culture radical scavenging percentage, the ‎content of chlorogenic acid and caffeic acid were significantly influenced by salinity and the ‎highest amount of them were recorded in the samples in which treated with 300 µM NaCl, ‎control and 600 µM NaCl, respectively. In indirect culture no significant difference was ‎observed on other phenolic compounds and antioxidant activity except caffeic acid.
 
DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.7.7

Keywords

Artichoke
Callus
Phenol
Flavonoid

Subjects

Journal of Ethno-Pharmaceutical Products
Volume 2, Issue 1
March 2021
Pages 45-51

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