Graduate University of Advanced Technology Journal of Ethno-Pharmaceutical Products 2383-3017 4 1 2024 12 01 Investigation of chemical and genetic diversity of Henna by using HPLC and ISSR marker بررسی تنوع شیمیایی و ژنتیکی حنا با استفاده ازHPLC و نشانگر ISSR 59 67 719837 10.48306/epp.2025.2045883.1068 EN Golrokh Kalantari Motamedi Graduate University of Advanced Technology, Kerman, Iran. Amin Baghizadeh Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. 0000-0001-5587-2621 Mahmood Maleki Dept. of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. 0000-0001-7184-3919 Masoud Torkzadeh Mahani Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. Journal Article 2024 11 16 Lawsonia inermis is utilized in the cosmetics industry and traditional medicine for the treatment of various ailments. Understanding the chemical and genetic diversity of this plant is essential for breeding purposes. This research investigated the chemical and genetic diversity of 12 distinct populations of Lawsonia inermis from different areas of Kerman province through the use of HPLC and ISSR markers. HPLC was employed to quantify the Lawson compound in the 12 populations, while three ISSR primers were utilized to evaluate genetic diversity. The findings showed notable diversity in Lawson content among samples collected from Jiroft, Shahdad, and Ghale Ganj, with the J5 sample (from Jiroft) displaying the highest value. Furthermore, the ISSR marker demonstrated that all populations could be grouped into three categories, with samples from the same region not necessarily clustering together. Although the marker could not differentiate populations based on Lawson content, it effectively distinguished them based on genetic diversity. Lawsonia inermis is utilized in the cosmetics industry and traditional medicine for the treatment of various ailments. Understanding the chemical and genetic diversity of this plant is essential for breeding purposes. This research investigated the chemical and genetic diversity of 12 distinct populations of Lawsonia inermis from different areas of Kerman province through the use of HPLC and ISSR markers. HPLC was employed to quantify the Lawson compound in the 12 populations, while three ISSR primers were utilized to evaluate genetic diversity. The findings showed notable diversity in Lawson content among samples collected from Jiroft, Shahdad, and Ghale Ganj, with the J5 sample (from Jiroft) displaying the highest value. Furthermore, the ISSR marker demonstrated that all populations could be grouped into three categories, with samples from the same region not necessarily clustering together. Although the marker could not differentiate populations based on Lawson content, it effectively distinguished them based on genetic diversity. https://js.kgut.ac.ir/article_719837_171615c24dd59f4f24ae7bdf75c4b29e.pdf