Graduate University of Advanced Technology Journal of Ethno-Pharmaceutical Products 2383-3017 2 1 2021 03 01 Sulforaphane production and antioxidant enzyme activity in Lepidium draba seedlings in presence of Yeast extract and silver nitrate 1 8 14371 EN Ali Riahi- Madvar Kerman Graduate University of Advanced Technology Solmaz Sarhady Department of Biotechnology, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran Mehdi Mohammadi Department of Biotechnology, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran Journal Article 2015 07 06 Sulforaphane is produced through a hydrolytic reaction catalyzed by myrosinase from  glucoraphanin. It has recently attracted researcher's interest due to its anticancer activities. The present study was conducted to evaluate the effects of various concentrations of yeast extract (YE) and silver ion (Ag⁺) on sulforaphane production level in <em>Lepidium draba</em> seedlings. Our results revealed that the sulforaphane content is increased in seedlings that treated with the both elicitors, but more significantly by Ag⁺. All elicitor influences are depend on the elicitor dose and time of elicitation and maximized with YE at 1 mg/mL for 8 hours and with Ag⁺ at 50 µM for 4 hours treatment. It seems that the promotion of sulforaphane content results in induction of glucoraphanin biosynthesis pathway which in turn is mediated by H₂O₂, as revealed by a significant increase in the activity of H₂O₂ scavenger enzymes.<br />  <br /> <strong><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">DOR:</span></span></strong><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> https://dorl.net/dor/<span id="MainContent_jRepeater_DOR_lblDOR_0" style="color: black;">20.1001.1.23833017.2021.2.1.1.1</span></span></span> https://js.kgut.ac.ir/article_14371_306b0c0a95972617146049ce1a9a1613.pdf