Journal of Ethno-Pharmaceutical Products

Abstract Lawsonia inermis is utilized in the cosmetics industry and traditional medicine for the treatment of various ailments. Understanding the chemical and genetic diversity of this plant is essential for breeding purposes. This research investigated the chemical and genetic diversity of 12 distinct populations of Lawsonia inermis from different areas of Kerman province through the use of HPLC and ISSR markers. HPLC was employed to quantify the Lawson compound in the 12 populations, while three ISSR primers were utilized to evaluate genetic diversity. The findings showed notable diversity in Lawson content among samples collected from Jiroft, Shahdad, and Ghale Ganj, with the J5 sample (from Jiroft) displaying the highest value. Furthermore, the ISSR marker demonstrated that all populations could be grouped into three categories, with samples from the same region not necessarily clustering together. Although the marker could not differentiate populations based on Lawson content, it effectively distinguished them based on genetic diversity.

Abstract Juniperus sabina L. is normally low shrub with procumbent or obliquely ascending branches, or rarely a small tree to about 4 m, monoecious or dioecious. Juniperus sabina L. is a medicinal plant used in folk medicine as an abortive. Its lignanes  have antineoplastic and antiviral activity, and also essential oil of Juniperus sabina L. has shown antibacterial and antifungal activity. The aim of this research is to extract and measure the chemical compounds of essential oils  of fruits and aerial parts of the plant Juniperus sabina L. which are collected from the  North Highlands located in the Mazandaran province. In this study, the components of Juniperus sabina L. (fruits and aerial parts),  after extraction essential oils by Clevenger apparatus, their compounds were analysed and identified by GC/MS. Essential oils from fruits of the Juniperus sabina L., were shown 27 compounds that the Sabinene in 50.31% maximum, and α-Thujene with 0.32% were the lowest amounts in the oils. While the essential oils from aerial parts of Juniperus sabina L. 40 compounds were identified, that Sabinene by 36.59% was the highest amount and α-Thujene by 0.11%  has at the lowest percentage.   DOR: https://dorl.net/dor/20.1001.1.23833017.2014.1.2.8.9

Abstract A. calamus that grows in province of Baluchistan and Kerman in Iran plays a major role in the revival of traditional medicine. Despite the useful role of this plant in many ailments, its anti-bacterial properties are not well understood. In this study, we investigated anti-bacterial activities of rhizomes ware of A. calamus against various strains of bacteria such as Staphylococcus aureus and Escherichia coli. The anti-microbial activity of extracts of A.calamus was assessed by disc diffusion method. Rhizomes extracted by ethanol, methanol solvents and essential oil were obtained by clevenger apparatus. Antibacterial effects of the extracts tested at concentrations (25, 100, 200, 250, 300, 400 mg/ml). The Minimal Inhibitory Concentration (MIC) of extracts ranged from 25-100 mg/ml against the susceptible bacteria. All the Minimal Bactericidal Concentrations (MBCs) were the same as the MICs. Our results revealed that ethanolic and methanolic extracts have an inhibitory effect on all gram positive and negative strains and it is comparable with kanamycin, an anti-bacterial reference drug. Amongst extracts of A.calamus, ethanolic extracts showed maximum inhibitory activity (16mm) against Staphylococcus epidermidis. So we can conclude that this plant has anti-bacterial properties on all gram positive and negative bacteria. The result can be related to the nature of the compounds found in this plant. The effective GC-MS method was performed for the determination of essential oil compounds of A.calamus. The Gas Chromatography and Mass Spectrometry (GC-MS) analysis revealed that the presence of phenyl propanoids, monoterpenes, sesquiterpenes and β-asarone in essential oil of the plant caused its antibacterial properties. Our result showed that the ethanolic and methanolic extracts of A. calamus could be useful for the development of effective treatment for the control of infectious diseases.

DOR: https://dorl.net/dor/20.1001.1.23833017.2014.1.1.1.0