Samaneh Rahamouz-Haghighi; malek hossein asadi; amin baghizadeh
Volume 2, Issue 2 , September 2021, , Pages 42-50
Abstract
Background: Trachyspermum ammi and Foeiniculum vulgare (Apiaceae) have been widely employed in traditional medicine to treat many diseases. Methods: The cytotoxic activity of methanolic extracts and essential oils of T.ammi and F.vulgare seeds on gastric cancer cell line (AGS) and Human Skeletal Muscle ...
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Background: Trachyspermum ammi and Foeiniculum vulgare (Apiaceae) have been widely employed in traditional medicine to treat many diseases. Methods: The cytotoxic activity of methanolic extracts and essential oils of T.ammi and F.vulgare seeds on gastric cancer cell line (AGS) and Human Skeletal Muscle cell line (HSkMC) were assessed by MTT method. In vitro toxicity was also evaluated on Artemia salina. The antibacterial activity was measured by the Microtiter broth dilution method. Result: The proliferation of cancer cells was inhibited by methanolic extracts and essential oils. The results showed a greater degree of cytotoxicity on AGS at the dose of 400μg/mL of methanolic extracts and essential oils of T.ammi and F.vulgare with IC50 values lower than 50 µg/mL at 48 to 72h. so, they can be considered appropriate for further purification and are agree with the US National Cancer Institute. The methanolic extracts and essential oils exhibited cytotoxicity activity against brine shrimp larvae (LC50: 1066.4 and 137.5 µg/mL for T.ammi) and (LC50: 1267.5 and 235.7 µg/mL for F.vulgare). The antimicrobial activity of methanolic extracts and essential oils showed maximum inhibitory activity against S.aureus with MICs (0.35 and 0.08 fold) and (0.45 and 0.1 fold) for T.ammi and F.vulgare, respectively. Conclusion: Therefore, our results showed that the methanolic extracts and essential oils of T.ammi and F.vulgare have antiproliferation and antibacterial properties and could be used as adjuvant therapy against common gastric and pathogenic bacteria.
Herbal Science
Rozita Kabiri; Mehdi Naghizadeh; Mohammad Javad Zarea
Volume 2, Issue 1 , March 2021, , Pages 9-16
Abstract
Compounds which are able to reduce damaging effects of various stresses such as drought could be of great importance. In this research, arginine was used as a precursor of nitric oxide or polyamines and the effect of this compound on alleviation of oxidative damages under drought stress has been investigated. ...
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Compounds which are able to reduce damaging effects of various stresses such as drought could be of great importance. In this research, arginine was used as a precursor of nitric oxide or polyamines and the effect of this compound on alleviation of oxidative damages under drought stress has been investigated. Experimental treatments included arginine at three levels (0, 10 and 20 µmol) and, drought stress (induced by polyethylene glycol 6000) at the levels of 0, 13.5% and 17% (W/V). This experiment was conducted as a completely randomized design in a factorial arrangement with three replications. The application of arginine through the root medium, increased drought tolerance of ajwain (Trachyspermum ammi) seedlings. Arginine profoundly induced the activities of phenylalanine ammonia-lyase and, polyphenol oxidase in plants which led to reduction in electrolyte leakage and increasing in relative water content, photosynthetic pigments (chlorophyll a, b, total chlorophyll and, carotenoids), polyphenol compounds, flavonoids, anthocyanin content and, soluble sugar content. It is concluded that the application of arginine appeared to induce pre-adaptive responses to drought stress, leading to promote protective reactions. DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.2.2