Medicinal Plant Chemistry
mostafa najar; Khodayar Hemmat; Amir Daraei Garmekhany; Aminallah Bagherifard
Volume 2, Issue 1 , March 2021, , Pages 29-37
Abstract
In this study the effect of altitude (in two provinces of Mazandaran and Golestan) on some morphological and biochemical properties of the stems, flowers and root of nettle plant was examined. Sampling was performed at the flowering time of plant. Extraction and identification of active ingredients were ...
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In this study the effect of altitude (in two provinces of Mazandaran and Golestan) on some morphological and biochemical properties of the stems, flowers and root of nettle plant was examined. Sampling was performed at the flowering time of plant. Extraction and identification of active ingredients were performed by using spectrophotometer and HPLC methods. This research was conducted based on completely randomized design by using statistical analysis software (SAS, 9.1). Results that altitude did not show significant effect on morphological properties, but in general it can be said that the height 1450m above the sea level in both provinces was the best height for morphological characteristics. Maximum size of stem diameter (7.05cm), root diameter (5.01cm), root length (31.1cm) and number of inflorescence (54.2) were observed in Golestan province at the altitude of 1450m and the highest size of inflorescence (5.52cm) in the Mazandaran province was related to the altitude of 1450m above the sea levels. The amount of total phenol, total flavonoids, Chlorogenic acid, caffeic acid and rutin in the stem and leaf, nettle root increased with increasing the height, so that the largest amount of total phenol (3.38 and 1.28 mg/g), total flavonoids (3.98 and 2.53 mg/g), Chlorogenic Acid (0.3 and 0.13 mg/g), caffeic acid (0.065 and 0.021 mg/g) and rutin (0.37 mg/g) were observed in stem, flower and root plant at altitude of 2250 m in leh koh region of Mazandaran province. Results showed that positive correlations exist between total phenol and flavonoid content and height of sea level. Many of morphological parameters such as stem diameter, root diameter, root length and number of inflorescence in Golestan province increased with increasing altitude up to 1450 m and the size of inflorescence in Mazandaran province in the same height showed the highest value. DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.5.5
Medicinal Plant Chemistry
Aminallah Bagherifard; Azim Ghasemnezhad; Abas Bagheri; Mojtaba Barani Motlagh; Abas Rezayiasl
Volume 2, Issue 1 , March 2021, , Pages 38-44
Abstract
The magnetic fields are one of the effective environmental factors on biological phenomena in living organisms especially plants. Artichoke (Cynara scolymus L.) is a medicinal plant that plays a good role in blood cholesterol reduction and liver disorders, this study has performed to investigate the ...
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The magnetic fields are one of the effective environmental factors on biological phenomena in living organisms especially plants. Artichoke (Cynara scolymus L.) is a medicinal plant that plays a good role in blood cholesterol reduction and liver disorders, this study has performed to investigate the effectiveness of magnetic water on growth, fresh and dry weight of leaf and root, efficiency of the water consuming and some chemical compounds of Artichoke plant in 2012. Experimental treatments include of four levels of electromagnetic field intensity (Zero, 3000, 6000 and 10000 Gauss) as a complete randomized design with four replications. Analysis of variance of data revealed that plant growth, shoot weight, root weight and efficiency of water consuming and chemical compounds were significantly influenced by irrigation with magnified water. The highest Phenol and Antioxidant activity of plant extract was observed in samples which were irrigated with water in which magnified with 6000G magnetic field with 3.99 mg/g and 66.74%, respectively. Contrast to that the highest amount of chlorogenic acid (0.20 mg/g) was observed in samples which were irrigated using top water. Based on the obtained results Therefore, it is concluded this level (6000G) of intensity has better effectiveness in comparison to other level on quality of Irrigation water and could increase quality Artichoke plant. DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.6.6
Medicinal Plant Chemistry
Masome Rahimbareshi; Azim Ghasemnezhad; Mehdi Alizadeh; Ahad Yamochi
Volume 2, Issue 1 , March 2021, , Pages 45-51
Abstract
Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ingredient is concerned by pharmaceutical industry. Abiotic stresses including salinity, changes the metabolic pathways of cultured cells and leads to the production of secondary metabolites. This study ...
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Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ingredient is concerned by pharmaceutical industry. Abiotic stresses including salinity, changes the metabolic pathways of cultured cells and leads to the production of secondary metabolites. This study was aimed to determine the effect of salt stress on antioxidant compounds of callus focusing on the phenol, flavonoid, and chlorogenic acid and caffeic acid production under in vitro conditions. The present study was carried out as two separate experiments as direct culture (cultured explant) on MS solid medium with different salinity concentrations (0, 50, 150, 300, 600, 1200 µM NaCl) and indirect culture (callus culture) in different salin concentrations of 0, 50, 150, 300, 600, 1200, 2000, 4000, 6000 and 75000 µM NaCl. Four weeks after culture the mentioned compounds were measured via distinctive methods. Results showed that, versus to indirect culture radical scavenging percentage, the content of chlorogenic acid and caffeic acid were significantly influenced by salinity and the highest amount of them were recorded in the samples in which treated with 300 µM NaCl, control and 600 µM NaCl, respectively. In indirect culture no significant difference was observed on other phenolic compounds and antioxidant activity except caffeic acid. DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.7.7