Medicinal Plant Chemistry
Masome Rahimbareshi; Azim Ghasemnezhad; Mehdi Alizadeh; Ahad Yamochi
Volume 2, Issue 1 , March 2021, , Pages 45-51
Abstract
Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ingredient is concerned by pharmaceutical industry. Abiotic stresses including salinity, changes the metabolic pathways of cultured cells and leads to the production of secondary metabolites. This study ...
Read More
Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ingredient is concerned by pharmaceutical industry. Abiotic stresses including salinity, changes the metabolic pathways of cultured cells and leads to the production of secondary metabolites. This study was aimed to determine the effect of salt stress on antioxidant compounds of callus focusing on the phenol, flavonoid, and chlorogenic acid and caffeic acid production under in vitro conditions. The present study was carried out as two separate experiments as direct culture (cultured explant) on MS solid medium with different salinity concentrations (0, 50, 150, 300, 600, 1200 µM NaCl) and indirect culture (callus culture) in different salin concentrations of 0, 50, 150, 300, 600, 1200, 2000, 4000, 6000 and 75000 µM NaCl. Four weeks after culture the mentioned compounds were measured via distinctive methods. Results showed that, versus to indirect culture radical scavenging percentage, the content of chlorogenic acid and caffeic acid were significantly influenced by salinity and the highest amount of them were recorded in the samples in which treated with 300 µM NaCl, control and 600 µM NaCl, respectively. In indirect culture no significant difference was observed on other phenolic compounds and antioxidant activity except caffeic acid. DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.7.7
Medicinal Plant Chemistry
Kianoush Hassanzadeh; Shiva Rahimi Tanha; Mohamad Mehdi Farzinia; Azim Ghasemnezhad
Volume 1, Issue 2 , September 2014, , Pages 55-60
Abstract
Rosmarinus officinalis is one of the most important medicinal and aromatic plants around the world. Essential oil as a weapon against biotic and abiotic stresses plays an important role in essential oil bearing plants. In this study the essential oil content and constituent of plants containing bacterium-gall ...
Read More
Rosmarinus officinalis is one of the most important medicinal and aromatic plants around the world. Essential oil as a weapon against biotic and abiotic stresses plays an important role in essential oil bearing plants. In this study the essential oil content and constituent of plants containing bacterium-gall on the shoots and crown was compared with non-contaminated plants. Essential oil percentage was extracted by hydro-distillation using Clevenger trap. Identification of the essential oil compounds was done by GC/MS. Results in essential oil percentage showed that, contaminated plants were relatively lower than that of non-contaminated plants per 100 grams of dry leaves. GC/MS also, verified that α- pinene and Camphor, respectively were 1.43% and 7.02%, more in contaminated plant than non-contaminated plant. In addition, Berbenone (18%), was the most common constituent which had been observed only in contaminated rosemaries. The analysis of leaf extract using HPLC, showed that the content of rosmarinic acid of healthy plants was significantly lower than that of contaminated plants. In addition, contaminated plants had total phenol and flavonoid compounds as well as antioxidant activity in the highest amount. Based on the obtained results, it can be concluded that, gall bacteria had influence on the phenolic components which are known as secondary metabolites in this medicinal plant and phenolic derivatives may be modified in quantity and quality by the affection of this biotic stressor, this could be considered as an application for further usage in metabolic culture.
DOR: https://dorl.net/dor/20.1001.1.23833017.2014.1.2.7.8