Investigation of chemical and genetic diversity of Henna by using HPLC and ISSR marker
Volume 4, Issue 1, March 2024, Pages 59-67
https://doi.org/10.48306/epp.2025.2045883.1068
Golrokh Kalantari Motamedi, Amin Baghizadeh, Mahmood Maleki, Masoud Torkzadeh Mahani
Abstract Lawsonia inermis is utilized in the cosmetics industry and traditional medicine for the treatment of various ailments. Understanding the chemical and genetic diversity of this plant is essential for breeding purposes. This research investigated the chemical and genetic diversity of 12 distinct populations of Lawsonia inermis from different areas of Kerman province through the use of HPLC and ISSR markers. HPLC was employed to quantify the Lawson compound in the 12 populations, while three ISSR primers were utilized to evaluate genetic diversity. The findings showed notable diversity in Lawson content among samples collected from Jiroft, Shahdad, and Ghale Ganj, with the J5 sample (from Jiroft) displaying the highest value. Furthermore, the ISSR marker demonstrated that all populations could be grouped into three categories, with samples from the same region not necessarily clustering together. Although the marker could not differentiate populations based on Lawson content, it effectively distinguished them based on genetic diversity.
Investigating the possibility of squalene production by some medicinal plants using thin-layer chromatography (TLC)
Volume 3, Issue 1, September 2023, Pages 24-30
https://doi.org/10.48306/epp.2023.2002740.1056
Mahmood Maleki, Omolbanin Nezhad Dehbakri, Davoud Darvishi Zeidabadi, Shahryar Shakeri
Abstract Medicinal plants contain active ingredients in one or some of their organs. Squalene is one of the active ingredients that prevent heart attacks and cardiovascular diseases and protect the body from some cancers. The aim of the present study was to investigate the presence of squalene in a number of medicinal plants. In this experiment, the plant oils were extracted and measured using Bligh & Dyer with minor changes. TLC (thin layer chromatography) was used to identify squalene. Comparison of TLC of standard squalene with TLC of the investigated medicinal plant samples showed that Caryophillium aromaticus, Descurainia sophia, Portulaca Oleracea, Papaver somniferum and Nigella Sativa contained squalene. Although the percentage of Papaver somniferum and Nigella Sativa seed oil was higher than other medicinal plants, the squalane spot of clove plant had a higher intensity of color and this indicates a higher concentration of squalene in this plant.