Interaction effect of size and type of zinc oxide nanoparticles with iron nanoparticles on increasing the growth of wheat plants under drought stress
Volume 4, Issue 1, March 2024, Pages 10-31
https://doi.org/10.48306/epp.2024.2033185.1066
Fatemeh AlSadat Amiri, Hossein Mozafari, hakimeh oloumi, Hassan Salari
Abstract Drought, as a multidimensional stress, has various destructive and adverse effects on plants and affects many morphological characteristics and physiological, biochemical and nutritional processes related to plant growth and development. This tension generates reactive oxygen species causes damage to the cell membrane. In the photosynthetic apparatus, the inhibition of electron transfer causes the reduction of the main photosynthetic pigments and protein accumulation. Wheat response to water deficit stress has many mechanisms that include cellular-molecular changes and its transfer to other metabolic activities and its effect on plant morphology. Therefore, the general purpose of this research was to investigate the interaction effect of iron oxide nanoparticles (25 nm) with two types of zinc nanoparticles (25 and 50 nm) at a concentration of 50 mg/liter on greater resistance of wheat (Arg cultivar) and improving its growth under drought stress conditions. Thus Wheat plants were treated with drought stress including two control levels and 7 days of water deficit. After applying drought treatments and the nanoparticles to the plants, some growth and pigments parameters were measured in stem, root and leaf to evaluate the effect of zinc and iron nanoparticles separately and mutually on the resistance of plants to drought stress. The occurrence of drought stress compared to the control led to a decrease in shoot length and other growth parameters. In this way, drought stress in the form of 5 days of water deprivation caused a decrease in root and stem length as well as other growth parameters such as fresh weight, dry weight, leaf water content, etc. compared to the control plants without drought stress. According to results, in conditions without drought stress, the application of both types of zinc oxide nanoparticles without or combined with iron oxide nanoparticles at the level of 5% had a significant effect.
Improving of drought stress by application of zinc and iron nanoparticles in tomato plant
Volume 4, Issue 1, March 2024, Pages 32-39
https://doi.org/10.48306/epp.2024.2040734.1067
hassan salari, moslem dehani, hossein mozafari
Abstract Drought stress affects many morphological characteristics and biochemical processes related to plant growth and development. In this sense, drought, as a multidimensional stress, has various destructive effects on plants. By inhibiting the transfer of electrons, this stress creates a secondary stress in the production of reactive oxygen species, causing double damage to the cell membrane, photosynthetic pigments, and other physiological parameters. Today, the use of metal nanoparticles such as zinc and iron in improving activities and dealing with plant stresses can create beneficial effects in plant and agricultural biotechnology. The treatments include drought stress at two levels (drought stress and no drought stress) as the main factor and three levels of iron, zinc separately and integrative of iron and zinc nanoparticles. The results showed that all investigated traits were affected by drought stress conditions, so that under drought stress the fresh and dry weight. Chlorophyll a and b, decreased and the usage of iron and zinc nanoparticles reduced the effects of drought stress in mentioned growth parameters in tomato plants. Therefore, the application of the above nanoparticles can be very effective in improving the growth of tomato plants, especially in dry areas.
EDTA increases the Phytoremediation and translocation factor of pb2+ in the medicinal plant Calendula officinalis
Volume 3, Issue 1, September 2023, Pages 17-23
https://doi.org/10.48306/epp.2023.2006198.1058
hassan salari, hossein mozafari
Abstract Phytoremediation of lead using plants in lead-contaminated soils is a new and safe environmental technology. By adding chelators and increasing plant extraction, the efficiency of this technology can be increased. In this regard, we evaluated the effect of adding EDTA chelates to lead-contaminated soils to investigate the amount of lead accumulation in a medicinal plant, Calendula officinalis. We designed a factorial experiment in the form of a completely randomized, with three replicates in pots and two factors including EDTA at two levels (0, 50 mg kg-1) and lead at four levels (0, 30, 90, and 270 mg kg-1). In this plant, the accumulation of lead was accompanied by an increase in the amount of lead in the soil due to the addition of EDTA to the soil. The results showed that EDTA significantly increased the lead translocation of lead from roots to the aerial part of the plant.
Total Chl. and shoot dry weight decrease significantly in EDTA treatment than control specific at a high level of Pb in the soil. Also, the results showed that EDTA increased lead removal from soil to soil solution and increased lead translocation from roots to the aerial part of the plant of Calendula officinalis. In general, the results of this research showed that with the careful management and EDTA use in lead extraction, it has provided a cost-effective and safe environmentally strategy.
Effect of iron oxide and zinc oxide nanoparticles on growth improvement and tolerance to salinity stress in tomato plants
Volume 3, Issue 1, September 2023, Pages 31-45
https://doi.org/10.48306/epp.2023.2011012.1062
hossein mozafari, Masoumeh Hejabi, hassan salari, hakimeh oloumi
Abstract More than 22% of the world's agricultural land is saline, and this trend continues to increase with climate changes. Salinity stress causes leaf color change, osmotic stress, ionic toxicity, prevents growth, photosynthesis and plant performance. Due to their size less than micron, metal nanoparticles have a great absorption and transmission power in plants. Salinity stress is a major problem in hot and dry areas under tomato cultivation. For this purpose, investigating the mutual effects of the size and type of zinc oxide and iron oxide nanoparticles on the improvement and change of growth and increasing the resistance to salt stress in tomato plants of the early urbana variety were carried out in the form of a completely randomized and factorial design with 4 replications, at a significant level of 5%. In this research, zinc oxide nanoparticles in 25 and 50 nm sizes, iron oxide in 25 nm sizes and sodium chloride in 0 and 75 mM levels were used. Nanoparticles and salinity treatments were both applied to the plants. The results showed that salt stress led to a decrease in plant growth parameters such as shoot and root length, leaf area, RWC, ion leakage. Also, NaCl led to an increase in the accumulation of prolin and other aldehydes, sodium, iron and zinc. The application of nanoparticles had a slight effect in stress-free conditions, but in stressed conditions, these two nanoparticles alone and especially in combination neutralized the effect of salinity and reduced the damage caused by salinity stress.