Phytochemistry
Ali Riahi- madvar; Solmaz Sarhady; Mehdi Mohammadi
Volume 2, Issue 1 , March 2021, , Pages 1-8
Abstract
Sulforaphane is produced through a hydrolytic reaction catalyzed by myrosinase from glucoraphanin. It has recently attracted researcher's interest due to its anticancer activities. The present study was conducted to evaluate the effects of various concentrations of yeast extract (YE) and silver ...
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Sulforaphane is produced through a hydrolytic reaction catalyzed by myrosinase from glucoraphanin. It has recently attracted researcher's interest due to its anticancer activities. The present study was conducted to evaluate the effects of various concentrations of yeast extract (YE) and silver ion (Ag+) on sulforaphane production level in Lepidium draba seedlings. Our results revealed that the sulforaphane content is increased in seedlings that treated with the both elicitors, but more significantly by Ag+. All elicitor influences are depend on the elicitor dose and time of elicitation and maximized with YE at 1 mg/mL for 8 hours and with Ag+ at 50 µM for 4 hours treatment. It seems that the promotion of sulforaphane content results in induction of glucoraphanin biosynthesis pathway which in turn is mediated by H2O2, as revealed by a significant increase in the activity of H2O2 scavenger enzymes. DOR: https://dorl.net/dor/20.1001.1.23833017.2021.2.1.1.1
Essential Oil Chemistry
Samaneh Rahamoz- Haghighi; Malek Hossein Asadi; Ali Riahi- madvar; Amin Baghizadeh
Volume 1, Issue 1 , March 2014, , Pages 1-7
Abstract
A. calamus that grows in province of Baluchistan and Kerman in Iran plays a major role in the revival of traditional medicine. Despite the useful role of this plant in many ailments, its anti-bacterial properties are not well understood. In this study, we investigated anti-bacterial activities of rhizomes ...
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A. calamus that grows in province of Baluchistan and Kerman in Iran plays a major role in the revival of traditional medicine. Despite the useful role of this plant in many ailments, its anti-bacterial properties are not well understood. In this study, we investigated anti-bacterial activities of rhizomes ware of A. calamus against various strains of bacteria such as Staphylococcus aureus and Escherichia coli. The anti-microbial activity of extracts of A.calamus was assessed by disc diffusion method. Rhizomes extracted by ethanol, methanol solvents and essential oil were obtained by clevenger apparatus. Antibacterial effects of the extracts tested at concentrations (25, 100, 200, 250, 300, 400 mg/ml). The Minimal Inhibitory Concentration (MIC) of extracts ranged from 25-100 mg/ml against the susceptible bacteria. All the Minimal Bactericidal Concentrations (MBCs) were the same as the MICs. Our results revealed that ethanolic and methanolic extracts have an inhibitory effect on all gram positive and negative strains and it is comparable with kanamycin, an anti-bacterial reference drug. Amongst extracts of A.calamus, ethanolic extracts showed maximum inhibitory activity (16mm) against Staphylococcus epidermidis. So we can conclude that this plant has anti-bacterial properties on all gram positive and negative bacteria. The result can be related to the nature of the compounds found in this plant. The effective GC-MS method was performed for the determination of essential oil compounds of A.calamus. The Gas Chromatography and Mass Spectrometry (GC-MS) analysis revealed that the presence of phenyl propanoids, monoterpenes, sesquiterpenes and β-asarone in essential oil of the plant caused its antibacterial properties. Our result showed that the ethanolic and methanolic extracts of A. calamus could be useful for the development of effective treatment for the control of infectious diseases. DOR: https://dorl.net/dor/20.1001.1.23833017.2014.1.1.1.0
Maryam Aminizadeh; Ali Riahi-Madvar; Mehdi Mohammadi
Volume 1, Issue 1 , March 2014, , Pages 8-14
Abstract
Sulforaphane (SFN) is an isothiocyanate which is produced through glucoraphanin hydrolysis via myrosinase activity. In the present study, SFN content and peroxidase activity has been assessed in treated Lepidium draba seedlings with different concentrations of iron and copper during 8 and 16 hours treatment. ...
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Sulforaphane (SFN) is an isothiocyanate which is produced through glucoraphanin hydrolysis via myrosinase activity. In the present study, SFN content and peroxidase activity has been assessed in treated Lepidium draba seedlings with different concentrations of iron and copper during 8 and 16 hours treatment. The results showed that the SFN content drastically elevated at treatment with 1 mg/L Fe2+ after 8 hours treatment which was around two-fold compared to the control. But its content decreased in treated seedlings with higher Fe2+ concentrations as well as treatment with all concentrations of Cu2+. Furthermore, promotion of peroxidase activity was seen in the treated seedlings with the both elicitors. The data revealed that not only the inhibitory effect of Cu2+ on SFN production was stronger than Fe2+, but its stimulatory effect on peroxidase activity was also remarkable especially after 16 hours treatment. It seems that the metals induce triggering of reactive oxygen species (ROS) which at low Fe2+ concentration leads to induce glucoraphanin biosynthesis pathway. While at higher Fe2+ concentrations as well as treatment seedlings with Cu2+, ROS lead to induce antioxidant enzyme system.
https://dorl.net/dor/20.1001.1.23833017.2014.1.1.2.1