Document Type: Research Paper
Municipality of Fereydunkenar
Department of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Islamic Azad University. Yasuj
In this study the effect of altitude (in two provinces of Mazandaran and Golestan) on some morphological and biochemical properties of the stems, flowers and root of nettle plant were examined. Sampling was performed at the flowering time of plant. Extraction and identification of active ingredients was performed by using spectrophotometer and HPLC methods. This research was conducted based on completely randomized design by using statistical analysis software (SAS, 9.1). Results of showed that altitude did not showed significant effect on morphological properties, but in general it can be said that the height1450 m above the sea level in both provinces was the best height for morphological characteristics. Maximum size of stem diameter (7.05cm), root diameter (5.01cm), root length (31.1cm) and number of inflorescence (54.2) were observed in Golestan province at the altitude of 1450 m and the highest size of inflorescence (5.52cm) in the Mazandaran province was related to the altitude of 1450 m above the sea levels. The amount of of total phenol , total flavonoids, Chlorogenic acid, caffeic acid and rutin in the stem and leaf, nettle root, increased with increase the height so that the largest amount of total phenol (3.38 and 1.28 mg/g), total flavonoids (3.98 and 2.53 mg/g), Chlorogenic Acid (0.3 and 0.13 mg/g), caffeic acid (0.065 and 0.021 mg/g) and rutin (0.37 mg/g) were observed at stem, flower and root plant at altitude of 2250 m in leh koh region of Mazandaran province. Results showed that positive correlations exist between total phenol and flavonid content and height of sea level. Many of morphological parameters such as stem diameter, root diameter, root length and number of inflorescence in Golestan province increased with increase altitude up to1450 m and the size of inflorescence in Mazandaran province in the same height showed the highest value