Study the effect of salt stress on antioxidant compounds of artichoke under invitro conditions

Document Type: Research Paper

Authors

1 Member of university

2 Msc

Abstract

Artichoke (Cynara scolymus) is one of the oldest herbs and nowadays because of its ‎ingredient is concerned by ‎pharmaceutical industry. Abiotic stresses including salinity, ‎changes the metabolic pathways of cultured cells and ‎leads to the production of secondary ‎metabolites. This study was aimed to determine the effect of salt stress on ‎antioxidant ‎compounds of callus focusing on the phenol, flavonoid, and chlorogenic acid and caffeic acid ‎production ‎under in vitro conditions. The present study was carried out as two separate ‎experiments as direct culture ‎‎(cultured explant) on MS solid medium with different salinity ‎concentrations (0, 50, 150, 300, 600, 1200 µM ‎NaCl) and indirect culture (callus culture) in ‎different salin concentrations of 0, 50, 150, 300, 600, 1200, ‎‎2000, 4000, 6000 and 75000 µM ‎NaCl. Four weeks after culture the mentioned compounds were measured ‎via distinctive ‎methods. Results showed that, versus to indirect culture radical scavenging percentage, the ‎content ‎of chlorogenic acid and caffeic acid were significantly influenced by salinity and the ‎highest amount of them were ‎recorded in the samples in which treated with 300 µM NaCl, ‎control and 600 µM NaCl, respectively. In indirect ‎culture no significant difference was ‎observed on other phenolic compounds and antioxidant activity except caffeic ‎acid.‎

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